8F60
anti-BTLA monoclonal antibody r23C8 in complex with BTLA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.464, 79.661, 74.692 |
Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.640 |
R-factor | 0.18272 |
Rwork | 0.181 |
R-free | 0.20546 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aw2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.258 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (v5.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.730 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.075 | 0.380 |
Number of reflections | 108178 | 15736 |
<I/σ(I)> | 8.3 | 2.3 |
Completeness [%] | 99.5 | 95.5 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 25% PEG 4K + 100mM Sodium Acetate pH 4.6 |