8F60
anti-BTLA monoclonal antibody r23C8 in complex with BTLA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.464, 79.661, 74.692 |
| Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.640 |
| R-factor | 0.18272 |
| Rwork | 0.181 |
| R-free | 0.20546 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aw2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.258 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.075 | 0.380 |
| Number of reflections | 108178 | 15736 |
| <I/σ(I)> | 8.3 | 2.3 |
| Completeness [%] | 99.5 | 95.5 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 25% PEG 4K + 100mM Sodium Acetate pH 4.6 |
