8EZG
Monobody 12D1 bound to KRAS(G12D)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 156.477, 156.477, 64.624 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.170 - 2.520 |
R-factor | 0.173 |
Rwork | 0.171 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 7L0G & 5VPZ |
RMSD bond length | 0.008 |
RMSD bond angle | 0.936 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.092 | 0.324 |
Rmeas | 0.098 | 0.344 |
Rpim | 0.033 | 0.113 |
Number of reflections | 16221 | 798 |
<I/σ(I)> | 20.3 | 7.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.7 | 9 |
CC(1/2) | 0.986 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.8 M sodium acetate trihydrate |