8EV7
Crystal structure of the Thermus thermophilus 70S ribosome in complex with kanamycin, mRNA, and A-, P-, and E-site tRNAs
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.363, 447.526, 617.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 152.879 - 2.890 |
R-factor | 0.2188 |
Rwork | 0.216 |
R-free | 0.26270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y4p |
RMSD bond length | 0.004 |
RMSD bond angle | 0.830 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 152.879 | 152.879 | 3.060 |
High resolution limit [Å] | 2.890 | 8.660 | 2.890 |
Rmerge | 0.265 | 0.032 | 2.075 |
Rmeas | 0.297 | 0.036 | 2.334 |
Total number of observations | 5874046 | ||
Number of reflections | 1264349 | 48100 | 195534 |
<I/σ(I)> | 6.81 | 34.45 | 0.8 |
Completeness [%] | 98.7 | 97.6 | 94.8 |
Redundancy | 4.646 | 4.377 | 4.687 |
CC(1/2) | 0.995 | 0.999 | 0.187 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME |