8EV1
Dual Modulators
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-07-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.055, 82.633, 58.553 |
| Unit cell angles | 90.00, 110.93, 90.00 |
Refinement procedure
| Resolution | 50.540 - 1.830 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qzo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.540 | 2.068 |
| High resolution limit [Å] | 1.828 | 1.828 |
| Rmerge | 0.123 | |
| Number of reflections | 27680 | 1385 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 92.6 | |
| Redundancy | 7.6 | |
| CC(1/2) | 0.998 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 20-25% PEG 3350, 200 mM MgCl2, 200 mM NaCl, 0.1 M Hepes pH 7 |
