8EI4
Crystal structure of the WWP1 HECT domain in complex with H302, a Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.181 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.420, 51.210, 58.640 |
| Unit cell angles | 112.09, 99.61, 102.51 |
Refinement procedure
| Resolution | 45.180 - 2.430 |
| R-factor | 0.2223 |
| Rwork | 0.219 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j1x |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.054 | 0.310 |
| Number of reflections | 16258 | 1472 |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 94.0 | 80.3 |
| Redundancy | 2.6 | 2.4 |
| CC(1/2) | 0.997 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M Sodium acetate, 0.1 M Sodium citrate pH 5.5, 5% w/v PEG 4000 |
