8EED

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ107-B in complex with ZIKV E glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-08
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	92.030, 105.630, 132.380
Unit cell angles	82.52, 70.48, 81.04

Refinement procedure

Resolution	48.842 - 3.487
R-factor	0.2906
Rwork	0.289
R-free	0.33060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nip
RMSD bond length	0.002
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	70659	5711
<I/σ(I)>	3.2
Completeness [%]	92.3
Redundancy	2.1
CC(1/2)	0.750	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15% PEG 6000, 5% MPD, 0.1 M MES (pH 6.5)