8EDF

Bovine Fab SKD in complex with Sars COV-2 receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 41 21 2
Unit cell lengths	162.841, 162.841, 125.171
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.740 - 3.400
R-factor	0.2771
Rwork	0.276
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6oo0
RMSD bond length	0.002
RMSD bond angle	0.641
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.740	3.550
High resolution limit [Å]	3.400	3.400
Rmerge	0.287	2.390
Rmeas	0.318	2.650
Rpim	0.135	1.120
Number of reflections	23014	1145
<I/σ(I)>	5.8	0.69
Completeness [%]	96.6	99.6
Redundancy	5	5.1
CC(1/2)	0.726	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293.15	0.4M Lithium chloride, 10% Peg6000, 0.1M sodium citrate