8EBM
Structure of KLHDC2 substrate binding domain bound to KLHDC2's C-degron mimic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.761, 88.634, 89.082 |
Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
Resolution | 39.410 - 1.580 |
R-factor | 0.1946 |
Rwork | 0.192 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6do2 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
Rmerge | 0.070 | 0.043 | 0.644 |
Rmeas | 0.084 | 0.052 | 0.766 |
Rpim | 0.046 | 0.030 | 0.410 |
Total number of observations | 296998 | ||
Number of reflections | 90061 | 8910 | 8993 |
<I/σ(I)> | 11.1 | ||
Completeness [%] | 97.8 | 95.4 | 98 |
Redundancy | 3.3 | 3 | 3.3 |
CC(1/2) | 0.994 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 8-12% PEG3350, 0.2M NaSCN, 0.1 M Bis-Tris Propane, 5 nM Hexamine cobalt (III) chloride, pH = 7.5 |