8EB0

RNF216/E2-Ub/Ub transthiolation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	C 1 2 1
Unit cell lengths	152.545, 70.363, 65.152
Unit cell angles	90.00, 108.70, 90.00

Refinement procedure

Resolution	41.370 - 3.030
R-factor	0.3062
Rwork	0.303
R-free	0.33890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 7M4O 1ubq 4q5e & 7M4M AlphaFold model
RMSD bond length	0.002
RMSD bond angle	0.486
Data reduction software	XDS (Nov 1, 2016 BUILT=20161205)
Data scaling software	Aimless (version 0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	3.210
High resolution limit [Å]	3.030	3.030
Rmerge	0.111	0.979
Number of reflections	12690	1986
<I/σ(I)>	5.6	0.9
Completeness [%]	98.4
Redundancy	3.4
CC(1/2)	0.994	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	293	2 M ammonium sulfate