8EB0

RNF216/E2-Ub/Ub transthiolation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 ubiquitin-protein ligase RNF216	polymer	277	31552.1	1	UniProt (Q9NWF9) In PDB	Homo sapiens (human)	RING finger protein 216,RING-type E3 ubiquitin transferase RNF216,Triad domain-containing protein 3,Ubiquitin-conjugating enzyme 7-interacting protein 1,Zinc finger protein inhibiting NF-kappa-B
2	B	Ubiquitin-conjugating enzyme E2 L3	polymer	157	18126.8	1	UniProt (P68036) Pfam (PF00179) In PDB	Homo sapiens (human)	E2 ubiquitin-conjugating enzyme L3,L-UBC,UbcH7,Ubiquitin carrier protein L3,Ubiquitin-conjugating enzyme E2-F1,Ubiquitin-protein ligase L3
3	C, D	Ubiquitin	polymer	76	8576.8	2	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)
4	A	ZINC ION	non-polymer		65.4	7	Chemie (ZN)
5	A, D	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)

Sequence modifications

A: 510 - 784 (UniProt: Q9NWF9)

PDB	External Database	Details
Gly 508	-	expression tag
Pro 509	-	expression tag
Ala 688	Cys 688	engineered mutation

B: 1 - 154 (UniProt: P68036)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Lys 86	Cys 86	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	66832.6
Non-Polymers*	Number of molecules	10
	Total formula weight	746.1
All*	Total formula weight	67578.6

*Water molecules are not included.