8E4X

Human Adenosine Deaminase Acting on dsRNA (ADAR2-R2D) bound to dsRNA containing a G:3-deaza dA pair adjacent to the target site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	169.914, 63.236, 142.646
Unit cell angles	90.00, 118.07, 90.00

Refinement procedure

Resolution	58.270 - 2.800
R-factor	0.1973
Rwork	0.195
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vff
Data reduction software	XDS
Data scaling software	Aimless (0.7.9)
Phasing software	PHENIX (1.18)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.270	58.270	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.064	0.022	1.175
Total number of observations	99409	3003	15009
Number of reflections	31664	947	4741
<I/σ(I)>	12	46.6	1.2
Completeness [%]	95.3	86.1	98.1
Redundancy	3.1	3.2	3.2
CC(1/2)	0.998	0.999	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	50 mM MOPS pH 7.0, 100 mM NaCl, 13% PEG 4000