8E4X
Human Adenosine Deaminase Acting on dsRNA (ADAR2-R2D) bound to dsRNA containing a G:3-deaza dA pair adjacent to the target site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.914, 63.236, 142.646 |
Unit cell angles | 90.00, 118.07, 90.00 |
Refinement procedure
Resolution | 58.270 - 2.800 |
R-factor | 0.1973 |
Rwork | 0.195 |
R-free | 0.23940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vff |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.9) |
Phasing software | PHENIX (1.18) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.270 | 58.270 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.064 | 0.022 | 1.175 |
Total number of observations | 99409 | 3003 | 15009 |
Number of reflections | 31664 | 947 | 4741 |
<I/σ(I)> | 12 | 46.6 | 1.2 |
Completeness [%] | 95.3 | 86.1 | 98.1 |
Redundancy | 3.1 | 3.2 | 3.2 |
CC(1/2) | 0.998 | 0.999 | 0.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 50 mM MOPS pH 7.0, 100 mM NaCl, 13% PEG 4000 |