8E1P
Crystal structure of BG505 SOSIP.v4.1-GT1.2 trimer in complex with gl-PGV20 and PGT124 Fabs
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 146.057, 157.666, 158.558 |
| Unit cell angles | 90.00, 102.97, 90.00 |
Refinement procedure
| Resolution | 49.760 - 3.820 |
| R-factor | 0.2723 |
| Rwork | 0.271 |
| R-free | 0.30110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w6d |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.531 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.760 | 3.880 |
| High resolution limit [Å] | 3.800 | 3.820 |
| Number of reflections | 63948 | 5699 |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 95.7 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.780 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M ammonium sulfate, 0.1 M Tris, pH 8.5, 12% w/v PEG8000 |
