8DY5
Crystal Structure of spFv CAT2200 LH in complex with IL-17A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.430, 226.380, 75.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.250 - 2.200 |
R-factor | 0.1902 |
Rwork | 0.188 |
R-free | 0.23240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qhu |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.260 | 45.260 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.120 | 0.032 | 1.012 |
Rmeas | 0.134 | 0.037 | 1.127 |
Number of reflections | 36090 | 452 | 2630 |
<I/σ(I)> | 10.27 | 30.33 | 1.98 |
Completeness [%] | 99.4 | 95 | 99.5 |
Redundancy | 5 | ||
CC(1/2) | 0.997 | 0.999 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 400 mM LiSO4, 15.5% PEG 3350 |