8DSF
Structure of cIAP1 with BCCov
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 28.990, 30.770, 118.630 |
Unit cell angles | 96.70, 90.22, 111.08 |
Refinement procedure
Resolution | 28.520 - 1.500 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kmn |
RMSD bond length | 0.008 |
RMSD bond angle | 0.850 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.519 | 1.495 |
High resolution limit [Å] | 1.391 | 1.391 |
Rpim | 0.037 | 0.428 |
Number of reflections | 47320 | 2367 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 72.4 | 27.3 |
Redundancy | 1.8 | |
CC(1/2) | 0.998 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.17 M Sodium Acetate, 0.085 M TrisBase pH 8.5, 25.5% w/v PEG 4000, 15% v/v glycerol |