8DJG
ADGRL3-lectin domain in complex with an activating synthetic antibody fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.799, 97.849, 163.058 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.510 | 2.400 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.038 | 7.310 |
Rmeas | 0.054 | 10.340 |
Rpim | 0.038 | 7.310 |
Number of reflections | 56710 | 4563 |
<I/σ(I)> | 9.3 | 0.1 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.990 | 0.079 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 296 | 100 mM sodium acetate, 150 mM ammonium sulfate, 20% PEG 4000 |