8DGQ

Crystal structure of p120RasGAP SH2-SH3-SH2 in complex with p190RhoGAP doubly phosphorylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	55.210, 113.500, 119.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.650 - 1.950
R-factor	0.215
Rwork	0.213
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsb 2j05
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.650	49.650	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.167	0.091	2.330
Rmeas	0.170	0.093	2.376
Rpim	0.034	0.019	0.462
Number of reflections	55390	5841	5492
<I/σ(I)>	12.37	29.92	1.94
Completeness [%]	100.0	100	100
Redundancy	25.459	24.194	26.029
CC(1/2)	0.998	0.998	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.5 M Sodium Malonate pH 5.0, 0.1 M sodium acetate trihydrate salt, 6% PEG 20,000