8DFQ
Ectodomain of full-length KIT(T417I,delta418-419)-SCF dimers
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.96 |
| Number of Particle | 2650475 |
| Software | cryoSPARC,PHENIX,EPU,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
