8DFG
Crystal structure of potently neutralizing human monoclonal antibody 42D6 Fab in complex with MSP1-19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.680, 71.370, 196.500 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.000 |
| R-factor | 0.2269 |
| Rwork | 0.225 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1OB1 & 3B2U |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.127 | 0.809 |
| Number of reflections | 75221 | 5165 |
| <I/σ(I)> | 6.03 | 1.61 |
| Completeness [%] | 96.3 | 91.1 |
| Redundancy | 3.247 | 2.985 |
| CC(1/2) | 0.994 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M Potassium chloride, 20 % PEG 3350 |
