8DF1

Chi3l1 bound by antibody C59

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	102.100, 172.920, 404.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.290 - 3.300
Rwork	0.206
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p8v 6peh 4hbc
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.290	3.418
High resolution limit [Å]	3.300	3.300
Rmerge	0.796
Number of reflections	108604	10701
<I/σ(I)>	8.97	1.87
Completeness [%]	99.9	99.8
Redundancy	11.8	11.7
CC(1/2)		0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1M Tris pH 8.5, 10% PEG 4000, 2% MPD