4HBC

Crystal structure of a conformation-dependent rabbit IgG Fab specific for amyloid prefibrillar oligomers

Replaces:  3NL4

Summary for 4HBC

• Entry DOI: 10.2210/pdb4hbc/pdb
• Descriptor: Antigen Binding Fragment, Immunoglobulin IgG - Heavy Chain, Antigen Binding Fragment, Immunoglobulin IgG - Light Chain, SULFATE ION, ... (4 entities in total)
• Functional Keywords: fab, rabbit, conformation-specific, amyloid, immune system
• Biological source: Oryctolagus cuniculus (rabbit); More
• Total number of polymer chains: 2
• Total formula weight: 45468.67

Authors

Arai, H. (deposition date: 2012-09-27, release date: 2012-10-31, Last modification date: 2024-11-06)

Primary citation

Arai, H.,Glabe, C.,Luecke, H.
Crystal structure of a conformation-dependent rabbit IgG Fab specific for amyloid prefibrillar oligomers.
Biochim.Biophys.Acta, 1820:1908-1914, 2012

PubMed Abstract: Although rabbit antibodies are widely used in research, no structures of rabbit antigen-binding fragments (Fab) have been reported. M204 is a rabbit monoclonal antibody that recognizes a generic epitope that is common to prefibrillar amyloid oligomers formed from many different amyloidogenic sequences. Amyloid oligomers are widely suspected to be a primary causative agent of pathogenesis in several age-related neurodegenerative diseases, such as Alzheimer's disease. The detailed structure of these amyloid oligomers is not known nor is the mechanism for the recognition of the generic epitope by conformation-dependent monoclonal antibodies.

PubMed: 22940003
DOI: 10.1016/j.bbagen.2012.08.016

Experimental method

X-RAY DIFFRACTION (1.54 Å)