4HBC
Crystal structure of a conformation-dependent rabbit IgG Fab specific for amyloid prefibrillar oligomers
Replaces: 3NL4Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979462 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.934, 42.184, 86.179 |
Unit cell angles | 90.00, 114.93, 90.00 |
Refinement procedure
Resolution | 31.000 - 1.540 |
R-factor | 0.2004 |
Rwork | 0.197 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.507 |
Data reduction software | d*TREK (9.7L) |
Data scaling software | d*TREK (9.7L) |
Phasing software | PHASER (1.3.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.010 | 31.010 | 1.600 |
High resolution limit [Å] | 1.540 | 3.320 | 1.540 |
Rmerge | 0.081 | 0.049 | 0.530 |
Total number of observations | 43516 | 14927 | |
Number of reflections | 56536 | ||
<I/σ(I)> | 10.2 | 25.4 | 2 |
Completeness [%] | 87.6 | 95.9 | 49.7 |
Redundancy | 6.46 | 6.7 | 4.67 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M Magnesium sulfate 20 % PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |