8DAF

Human SF-1 LBD bound to synthetic agonist 6N-10CA and bacterial phospholipid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	73.428, 73.428, 194.184
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.590 - 2.590
R-factor	0.2255
Rwork	0.223
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zdt
RMSD bond length	0.006
RMSD bond angle	0.696
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.590	2.680
High resolution limit [Å]	2.590	2.590
Rpim	0.035	0.453
Number of reflections	19623	1878
<I/σ(I)>	13.5
Completeness [%]	99.8
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	Na acetate (pH 4.6), glycerol, PEG 4000