8DAA
Coevolved affibody-Z domain pair LL2.c7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 49.588, 53.023, 68.662 |
Unit cell angles | 90.00, 98.39, 90.00 |
Refinement procedure
Resolution | 23.480 - 1.750 |
R-factor | 0.2558 |
Rwork | 0.251 |
R-free | 0.29850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5djt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.460 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.480 | 1.813 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.211 | 1.973 |
Rmeas | 0.244 | 2.297 |
Rpim | 0.121 | 1.158 |
Number of reflections | 17068 | 1711 |
<I/σ(I)> | 6.49 | 0.61 |
Completeness [%] | 95.2 | 95.52 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.991 | 0.356 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 3.0 M Ammonium sulfate, 100 mM HEPES pH 7.0 cryoprotected with sodium malonate |