5DJT

Crystal structure of LOV2 (C450A) domain in complex with Zdk2

Summary for 5DJT

• Entry DOI: 10.2210/pdb5djt/pdb
• Descriptor: NPH1-2, Engineered protein, Zdk2 affibody, COPPER (II) ION, ... (6 entities in total)
• Functional Keywords: protein binding, protein engineering, signaling protein, photoswitch, light-induced signal transduction, lov2, affibody, complex
• Biological source: Avena sativa (Oat); More
• Total number of polymer chains: 2
• Total formula weight: 23861.52

Authors

Tarnawski, M.,Wang, H.,Yumerefendi, H.,Hahn, K.M.,Schlichting, I. (deposition date: 2015-09-02, release date: 2016-07-20, Last modification date: 2024-01-10)

Primary citation

Wang, H.,Vilela, M.,Winkler, A.,Tarnawski, M.,Schlichting, I.,Yumerefendi, H.,Kuhlman, B.,Liu, R.,Danuser, G.,Hahn, K.M.
LOVTRAP: an optogenetic system for photoinduced protein dissociation.
Nat.Methods, 13:755-758, 2016

PubMed Abstract: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

PubMed: 27427858
DOI: 10.1038/nmeth.3926

Experimental method

X-RAY DIFFRACTION (1.4 Å)