8DAA

Coevolved affibody-Z domain pair LL2.c7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Immunoglobulin G-binding protein A	polymer	67	7761.5	2	UniProt (P38507) Pfam (PF02216) UniProt (by SIFTS) (P02976)	Staphylococcus aureus	IgG-binding protein A,Staphylococcal protein A,SpA
2	B, D (B, D)	Affibody LL2.FIVK	polymer	67	7632.6	2		synthetic construct
3	E (A)	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
4	F, G, H, I (A, B, C, D)	water	water		18.0	33	Chemie (HOH)

Sequence modifications

A, C: 2 - 58 (UniProt: P38507)

PDB	External Database	Details
Ala 0	-	expression tag
Val 1	-	expression tag
Leu 9	Gln 220	engineered mutation
Val 13	Phe 224	engineered mutation
Ala 29	Gly 240	engineered mutation
Phe 31	Ile 242	engineered mutation
Leu 59	-	expression tag
Glu 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	30788.2
Non-Polymers*	Number of molecules	1
	Total formula weight	102.0
All*	Total formula weight	30890.3

*Water molecules are not included.