8CYI
Cryo-EM structures and computational analysis for enhanced potency in MTA-synergic inhibition of human protein arginine methyltransferase 5
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.14 |
| Number of Particle | 866240 |
| Software | cisTEM,PHENIX,ISOLDE,Leginon,CTFFIND |
| Symmetry | D2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
