8CVL

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMTHSMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.30A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.430, 447.700, 618.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	121.530 - 2.300
R-factor	0.2396
Rwork	0.237
R-free	0.28140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.861
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	121.530	121.530	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.146	0.043	1.410
Rmeas	0.172	0.050	1.673
Total number of observations	8504865
Number of reflections	2467641	28381	175364
<I/σ(I)>	6.54	20.12	0.83
Completeness [%]	97.5	95.5	94
Redundancy	3.447	3.663	3.399
CC(1/2)	0.988	0.996	0.125

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME