8CRE

Crystal structure of the Candida albicans 80S ribosome in complex with geneticin G418

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2022-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	299.180, 294.180, 450.090
Unit cell angles	90.00, 100.05, 90.00

Refinement procedure

Resolution	162.010 - 3.000
R-factor	0.2642
Rwork	0.264
R-free	0.28070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	162.010	162.010	3.050
High resolution limit [Å]	3.000	50.000	3.000
Rmerge	0.641	0.052	2.847
Rmeas	0.664	0.055	2.950
Rpim	0.173
Number of reflections	1513706	286	72995
<I/σ(I)>	4.55
Completeness [%]	99.3
Redundancy	14.5
CC(1/2)	0.993	0.999	0.047

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside, geneticin G418