8CN9
Factor VII binding Fab of the bispecific antibody HMB-001 in complex with Factor VII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-08 |
| Detector | DECTRIS PILATUS3 R 1M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 144.782, 100.794, 181.739 |
| Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
| Resolution | 48.500 - 3.400 |
| R-factor | 0.3047 |
| Rwork | 0.303 |
| R-free | 0.35570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.494 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 3.522 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.310 | 1.050 |
| Rmeas | 0.350 | 1.210 |
| Rpim | 0.150 | |
| Number of reflections | 69059 | 6423 |
| <I/σ(I)> | 3.6 | 0.86 |
| Completeness [%] | 96.3 | 91.4 |
| Redundancy | 4.9 | 3.7 |
| CC(1/2) | 0.980 | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.15 M Cesium chloride 15% w/v Polyethylene glycol 3,350 |
