8CEB
Type2 alpha-synuclein filament assembled in vitro by wild-type and mutant (7 residues insertion) protein
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.8 |
| Software | RELION,EPU,Coot,REFMAC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
