8CAF
N8C_Fab3b in complex with NEDD8-CUL1(WHB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.372, 106.871, 180.651 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.330 - 2.660 |
R-factor | 0.2189 |
Rwork | 0.217 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.177 |
Data reduction software | XDS (Version November 3, 2014) |
Data scaling software | Aimless |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 180.000 | 2.740 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.055 | 1.165 |
Number of reflections | 56730 | 4609 |
<I/σ(I)> | 23.8 | |
Completeness [%] | 98.6 | |
Redundancy | 6.7 | |
CC(1/2) | 0.999 | 0.612 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0-2.1M Ammonium Sulfate, 0.1M Citrate pH 6.0, 10mM TCEP |