8BX2
Oct4/Sox2 protein:DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97624 |
Spacegroup name | P 63 |
Unit cell lengths | 163.169, 163.169, 39.792 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 81.720 - 3.140 |
R-factor | 0.20075 |
Rwork | 0.199 |
R-free | 0.25234 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ht5 |
RMSD bond length | 0.009 |
RMSD bond angle | 2.508 |
Data reduction software | XDS (20200417) |
Data scaling software | Aimless (0.5.27) |
Phasing software | MOLREP (11.7.03) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.585 | 3.342 |
High resolution limit [Å] | 3.137 | 3.137 |
Rmerge | 0.114 | 2.135 |
Number of reflections | 8506 | 424 |
<I/σ(I)> | 14.8 | 1.6 |
Completeness [%] | 77.4 | 22.7 |
Redundancy | 19.5 | 18.4 |
CC(1/2) | 0.996 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 70% (v/v) MPD |