8BDS
Ternary complex between VCB, BRD4-BD1 and PROTAC 48
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 79.031, 79.031, 204.206 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.443 | 1.871 |
High resolution limit [Å] | 1.718 | 1.718 |
Rmerge | 0.076 | 1.399 |
Rmeas | 0.081 | 1.481 |
Rpim | 0.026 | 0.483 |
Total number of observations | 593475 | |
Number of reflections | 63052 | 3152 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 95.9 | 69.1 |
Redundancy | 9.4 | 9.3 |
CC(1/2) | 0.999 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.4M Ammonium Sulfate, 4% (w/v) glycerol, 0.1M HEPES pH 7.5 |