8BCD
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 50
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.094, 121.921, 186.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.690 - 3.500 |
| R-factor | 0.2254 |
| Rwork | 0.223 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6s8q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.687 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.710 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmeas | 0.207 | |
| Number of reflections | 29691 | 4699 |
| <I/σ(I)> | 8.1 | 0.7 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.998 | 0.304 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350 |
