8B7W

Complex IL-17A/anti-IL-17A-76

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-08
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 31 2 1
Unit cell lengths	71.050, 71.050, 106.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.170 - 2.850
R-factor	0.2237
Rwork	0.222
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hr9 6rbb
RMSD bond length	0.010
RMSD bond angle	1.137
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.920
High resolution limit [Å]	2.850	2.850
Rmeas	0.270	1.970
Number of reflections	7466	538
<I/σ(I)>	12	1.58
Completeness [%]	98.1	99.4
Redundancy	6.7
CC(1/2)	0.960	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	1.05 M NaCl, 7% v/v ethanol, 30% glycerol.