8B6Q
X-ray structure of the haloalkane dehalogenase HaloTag7 with an insertion of Calmodulin-M13 fusion at position 154-156 that mimic the structure of CaProLa, an calcium gated protein labeling technology
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99989 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 74.530, 74.530, 177.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.106 | 0.851 |
Number of reflections | 16118 | 1676 |
<I/σ(I)> | 12.78 | |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 9.2 | 9.9 |
CC(1/2) | 0.998 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M MES pH 6.0, 0.2 M calcium acetate, 18% (m/v) PEG 8000 |