8B6Q
X-ray structure of the haloalkane dehalogenase HaloTag7 with an insertion of Calmodulin-M13 fusion at position 154-156 that mimic the structure of CaProLa, an calcium gated protein labeling technology
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Haloalkane dehalogenase,Calmodulin-1,Haloalkane dehalogenase,Calmodulin-1,M13 peptide | polymer | 472 | 52940.6 | 1 | UniProt (P0A3G3) UniProt (P0DP23) Pfam (PF00561) Pfam (PF13499) In PDB | Rhodococcus sp. (in: high G+C Gram-positive bacteria) | |
| 2 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 3 | A | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
| 4 | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A: 2 - 152 (UniProt: P0A3G3)
A: 156 - 301 (UniProt: P0DP23)
A: 307 - 328 (PDB: 8B6Q)
A: 334 - 467 (UniProt: P0A3G3)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Val 45 | Leu 47 | engineered mutation |
| Thr 56 | Ser 58 | engineered mutation |
| Gly 76 | Asp 78 | engineered mutation |
| Phe 85 | Tyr 87 | engineered mutation |
| Met 86 | Leu 88 | engineered mutation |
| Phe 126 | Cys 128 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 214 | Asn 61 | engineered mutation |
| Val 297 | Gln 144 | engineered mutation |
| Gly 302 | - | linker |
| Gly 303 | - | linker |
| Thr 304 | - | linker |
| Gly 305 | - | linker |
| Gly 306 | - | linker |
| Leu 329 | - | linker |
| Glu 330 | - | linker |
| Gly 331 | - | linker |
| Gly 332 | - | linker |
| Ser 333 | - | linker |
A: 334 - 467 (UniProt: P0A3G3)
| PDB | External Database | Details |
|---|---|---|
| Thr 153 | - | linker |
| Gly 154 | - | linker |
| Ser 155 | - | linker |
| Lys 337 | Glu 160 | engineered mutation |
| Val 344 | Ala 167 | engineered mutation |
| Thr 349 | Ala 172 | engineered mutation |
| Met 352 | Lys 175 | engineered mutation |
| Gly 353 | Cys 176 | engineered mutation |
| Asn 372 | Lys 195 | engineered mutation |
| Glu 401 | Ala 224 | engineered mutation |
| Asp 404 | Asn 227 | engineered mutation |
| Lys 434 | Glu 257 | engineered mutation |
| Ala 441 | Thr 264 | engineered mutation |
| Asn 449 | His 272 | engineered mutation |
| Leu 450 | Tyr 273 | engineered mutation |
| Ser 468 | - | expression tag |
| Thr 469 | - | expression tag |
| Leu 470 | - | expression tag |
| Glu 471 | - | expression tag |
| Ile 472 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 52940.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 275.9 | |
| All* | Total formula weight | 53216.5 |
