8AV9
INDUCED MYELOID LEUKEMIA CELL DIFFERENTIATION PROTEIN FABCOMPLEX IN COMPLEX WITH COMPOUND 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.284, 41.945, 106.071 |
| Unit cell angles | 90.00, 112.72, 90.00 |
Refinement procedure
| Resolution | 70.580 - 1.990 |
| R-factor | 0.2253 |
| Rwork | 0.223 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.580 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.079 | 0.577 |
| Number of reflections | 41926 | 41926 |
| <I/σ(I)> | 6.7 | 1.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 310 | 15% Peg2kMME, PCTP 0.1M pH 6.5 |
