8AHC

Crystal structure of the BRD9 bromodomain with BI-7189

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	69.883, 125.201, 29.463
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.600 - 1.504
R-factor	0.2121
Rwork	0.211
R-free	0.23000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5f1h
RMSD bond length	0.008
RMSD bond angle	0.760
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.600	1.672
High resolution limit [Å]	1.504	1.504
Number of reflections	25083	1254
<I/σ(I)>	19.4
Completeness [%]	59.5
Redundancy	6.3
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.44	277	100 mM Tris 28% glycerol ethoxylate