8ACF
Structure of the argX-117 in complex with a complement C2 fragment at low pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.830, 89.140, 146.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.570 - 1.800 |
R-factor | 0.1788 |
Rwork | 0.178 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ani |
RMSD bond length | 0.014 |
RMSD bond angle | 1.112 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.570 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.069 | 2.457 |
Number of reflections | 87785 | 8651 |
<I/σ(I)> | 19.2 | |
Completeness [%] | 100.0 | 99.98 |
Redundancy | 13.4 | 13.6 |
CC(1/2) | 1.000 | 0.472 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 50% MPD, 100 mM Hepes pH 5.5-6.0 |