8A5W

Crystal structure of the human phosphoserine aminotransferase (PSAT) in complex with O-phosphoserine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	65.433, 205.742, 135.750
Unit cell angles	90.00, 97.88, 90.00

Refinement procedure

Resolution	51.440 - 2.780
R-factor	0.1844
Rwork	0.182
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e77
RMSD bond length	0.005
RMSD bond angle	0.688
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.470	3.110
High resolution limit [Å]	2.780	2.780
Number of reflections	60488	3024
<I/σ(I)>	8.9
Completeness [%]	93.2
Redundancy	6.8
CC(1/2)	0.993	0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Na2SO4, 0.1 M Bis Tris Propane, pH 7,5, 20 % PEG 3350