8A0G

Human deoxyhypusine synthase with trapped transition state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.3
Synchrotron site	BESSY
Beamline	14.3
Temperature [K]	293
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.895
Spacegroup name	P 32 2 1
Unit cell lengths	106.950, 106.950, 161.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.520 - 1.840
R-factor	0.1475
Rwork	0.147
R-free	0.16410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xxj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.520	46.520	1.950
High resolution limit [Å]	1.840	5.480	1.840
Rmerge	0.192	0.062	1.967
Rmeas	0.203	0.066	2.072
Total number of observations	922420
Number of reflections	93129	3764	14866
<I/σ(I)>	8.58	30	0.97
Completeness [%]	99.9	99.6	99.5
Redundancy	9.905	8.953	10.101
CC(1/2)	0.997	0.998	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 mg/ml protein solution was mixed with equal volume of mother liquor consisting of 0.025-0.125 mM carboxylic acid mix, 30-60% precipitant mix, PEG 1000, PEG 3350 and 100 mM Tris-Bicine pH 8.5.