8YX1

CD40 in complex with Bleselumab Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	49.565, 116.002, 121.651
Unit cell angles	90.00, 91.71, 90.00

Refinement procedure

Resolution	45.560 - 2.700
Rwork	0.204
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.560
Data reduction software	XDS (20210323)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.560	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.143	0.941
Rpim	0.059	0.537
Number of reflections	37822	4604
<I/σ(I)>	12.8	2.3
Completeness [%]	99.9	100
Redundancy	6.8	6.9
CC(1/2)	0.996	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M Ammonium chloride 20% w/v Polyethylene glycol 3,350