8YAT

SOD1, Nanobody1, Nanobody2 and Nanobody3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	377.129, 46.672, 156.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.820 - 3.940
R-factor	0.3082
Rwork	0.303
R-free	0.36770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.820	4.150
High resolution limit [Å]	3.940	3.940
Rmerge	0.541	1.304
Rmeas	0.564	1.356
Rpim	0.156	0.369
Total number of observations	331414	49500
Number of reflections	25865	3680
<I/σ(I)>	5.6	2.4
Completeness [%]	99.9
Redundancy	12.8	13.5
CC(1/2)	0.981	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M Ammonium sulfate, 0.1M HEPES pH8.0, 20%w/v Polyethylene glycol 3350