Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.340, 49.630, 66.740 |
| Unit cell angles | 90.00, 106.94, 90.00 |
Refinement procedure
| Resolution | 26.140 - 1.750 |
| R-factor | 0.1884 |
| Rwork | 0.186 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wcl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.037 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.140 | 1.813 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.132 | 1.671 |
| Rmeas | 0.144 | |
| Number of reflections | 64921 | 6382 |
| <I/σ(I)> | 12.08 | 2.7 |
| Completeness [%] | 97.1 | 95.93 |
| Redundancy | 6.5 | 6.5 |
| CC(1/2) | 0.997 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18% PEG 8000, 0.2 M Li2SO4, and 0.1 M MES, pH 6.5 |
