8W50
Crystal structure of DNA binding and cleavage core of human topoisomerase 2-alpha in a DNA binding-competent conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 266.104, 266.104, 172.812 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.190 - 2.670 |
R-factor | 0.2224 |
Rwork | 0.222 |
R-free | 0.24780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.551 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.190 | 2.720 |
High resolution limit [Å] | 2.670 | 2.670 |
Rpim | 0.038 | |
Number of reflections | 128160 | 6358 |
<I/σ(I)> | 16.99 | |
Completeness [%] | 98.9 | |
Redundancy | 2 | |
CC(1/2) | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M ammonium sulfate, 0.1M citrate (pH5.5), 12% PEG 4000 |