8W50
Crystal structure of DNA binding and cleavage core of human topoisomerase 2-alpha in a DNA binding-competent conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 266.104, 266.104, 172.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.190 - 2.670 |
| R-factor | 0.2224 |
| Rwork | 0.222 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.551 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.190 | 2.720 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rpim | 0.038 | |
| Number of reflections | 128160 | 6358 |
| <I/σ(I)> | 16.99 | |
| Completeness [%] | 98.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M ammonium sulfate, 0.1M citrate (pH5.5), 12% PEG 4000 |
