8W0V

Crystal structure of broadly neutralizing antibody hcab55 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-06-29
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	67.019, 88.840, 175.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.670 - 2.590
R-factor	0.2049
Rwork	0.202
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.109
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.840	2.710
High resolution limit [Å]	2.590	2.590
Rmerge	0.166	1.210
Rmeas	0.183	1.335
Rpim	0.076	0.554
Total number of observations	183914	22282
Number of reflections	32994	3974
<I/σ(I)>	6.8	1.7
Completeness [%]	99.0
Redundancy	5.6	5.6
CC(1/2)	0.982	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	298	0.2 M lithium citrate tribasic tetrahydrate and 20% PEG 3,350