8VUI

Structure of FabS1CE-EPR-1, an elbow-locked Fab, in complex with the erythropoeitin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	56.390, 71.930, 197.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.510 - 2.100
R-factor	0.1991
Rwork	0.197
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.943
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.510	2.120
High resolution limit [Å]	2.060	2.060
Rmerge	0.107	1.193
Rmeas	0.117	1.298
Rpim	0.046	0.506
Number of reflections	46837	2699
<I/σ(I)>	9.3	1.9
Completeness [%]	92.5	69.7
Redundancy	6.5	6.6
CC(1/2)	0.995	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	298	0.2 M Ammonium Citrate dibasic, 20% PEG2000 monomethyl ether, pH 5.2