8VTV
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-91, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 211.850, 451.310, 630.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 122.660 - 2.550 |
R-factor | 0.2217 |
Rwork | 0.220 |
R-free | 0.26160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.839 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 122.660 | 122.660 | 2.620 |
High resolution limit [Å] | 2.550 | 11.400 | 2.550 |
Rmerge | 0.193 | 0.052 | 1.818 |
Rmeas | 0.215 | 0.058 | 2.029 |
Total number of observations | 9557286 | ||
Number of reflections | 1921762 | 22148 | 141597 |
<I/σ(I)> | 7.04 | 21.13 | 0.98 |
Completeness [%] | 99.4 | 97.1 | 99.4 |
Redundancy | 4.973 | 5.365 | 4.825 |
CC(1/2) | 0.991 | 0.995 | 0.199 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |