8VTU

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-66, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.980, 450.780, 623.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.270 - 2.400
R-factor	0.2231
Rwork	0.221
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.844
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	107.270	107.270	2.460
High resolution limit [Å]	2.400	10.740	2.400
Rmerge	0.157	0.037	1.681
Rmeas	0.172	0.040	1.854
Total number of observations	13552402
Number of reflections	2244913	26198	154136
<I/σ(I)>	10.06	39.08	0.96
Completeness [%]	98.9	98.1	92.1
Redundancy	6.037	6.206	5.466
CC(1/2)	0.995	0.998	0.167

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME